Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins

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Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins.

This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We an...

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ژورنال

عنوان ژورنال: Mol. BioSyst.

سال: 2013

ISSN: 1742-206X,1742-2051

DOI: 10.1039/c2mb25470k